Study on Liquid-Phase Hybridization in PCR-ELISA for the Detection of Genetically Modified Organisms

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Study on Liquid-Phase Hybridization in PCR-ELISA for the Detection of Genetically Modified Organisms
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Optimizing ingredient of hybridization solution and concentration of probes was conducted. 5'-biotinylated PCR product was automatically hybridized to a digoxigenin-labeled probe presented in the PCR reaction. The hybridization is performed as part of the

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LIU Guang-ming, LONG Ming-nan, SONG Si-Mang, SU Wen-jin. Study on Liquid-Phase Hybridization in PCR-ELISA for the Detection of Genetically Modified Organisms[J]. Journal of Food Science and Biotechnology,2003,22(2).

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