阿胶-燕窝复配物对孕期缺锌小鼠及子代生长发育与免疫功能的协同改善作用

Synergistic Ameliorative Effects of an Ejiao-Edible Bird’s Nest Compound on Growth, Development, and Immune Function of Zinc-deficient Pregnant Mice and Their Offspring

摘要:

【目的】探究阿胶-燕窝复配物对孕期缺锌合并应激所致孕鼠及仔鼠生长发育及免疫功能的协同改善作用。【方法】构建孕鼠缺锌模型，给予阿胶、燕窝及其复配物干预，测定母仔体质量、血清锌与免疫球蛋白水平，并进行脾脏免疫组化分析。【结果】阿胶-燕窝复配物可有效恢复母仔血清锌稳态（孕鼠最高达18.02 μmol/L），缺锌孕鼠体质量增长率显著提升至46.77%，且仔鼠早期生长迟缓得到缓解。在免疫调节方面，阿胶-燕窝复配物可特异性改善缺锌仔鼠血清免疫球蛋白A匮乏，其质量浓度升至1.20 mg/mL以上，并显著下调仔鼠异常升高的脾脏NK细胞及巨噬细胞标志物表达水平，抑制炎症反应。【结论】阿胶-燕窝复配物通过重构锌营养谱、增强黏膜免疫储备及双向调节脾脏免疫微环境，协同改善缺锌孕鼠及仔鼠的生长发育与免疫屏障损伤。

Abstract:

[Objective]This study aimed to investigate the synergistic ameliorative effects of an Ejiao-edible bird’s nest compound on the growth, development, and immune function of zinc-deficient pregnant mice and their offspring under stress.[Method]A pregnant mouse model of zinc deficiency was established and subjected to nutritional interventions with Ejiao, edible bird’s nest, and their compound. Body mass, serum zinc concentrations, and immunoglobulin levels in both pregnant mice and offspring were measured. Immunohistochemical analysis of the spleen was conducted.[Result]The compound effectively restored serum zinc homeostasis in both pregnant mice and offspring (with the serum zinc concentration reaching a maximum of 18.02 μmol/L in pregnant mice). It significantly increased the body mass gain rate of zinc-deficient pregnant mice to 46.77% and mitigated early growth retardation in the offspring. Regarding immunomodulation, the compound specifically reversed the severe deficit of serum immunoglobulin A in zinc-deficient offspring, increasing the mass concentration to over 1.20 mg/mL. Moreover, it significantly downregulated the abnormally elevated expression levels of NK cell and macrophage markers in the offspring’s spleen, thereby suppressing excessive inflammatory responses.[Conclusion]The Ejiao-edible bird’s nest compound synergistically ameliorates impairments in growth, development, and immune barrier function in zinc-deficient pregnant mice and offspring by reconstructing the zinc nutritional profile, enhancing mucosal immune reserves, and bidirectionally regulating the splenic immune microenvironment.