黑木耳蛋白质-多糖复合体系构建负载白藜芦醇的Pickering乳液及特性分析

Construction and Characterization of Resveratrol-loaded Pickering Emulsions Based on Auricularia auricula Protein-polysaccharide Complex

摘要:

【目的】以黑木耳（Auricularia auricula）为原料制备黑木耳蛋白质-多糖复合物（APPC），以此为基础制备负载白藜芦醇的Pickering乳液并对其进行结构表征，探究APPC负载白藜芦醇的Pickering乳液的稳定性及作用机制。【方法】采用水提醇沉法制备APPC，并对其组成和结构进行分析；进一步制备负载白藜芦醇的Pickering乳液，通过测定Zeta电位、三相接触角、流变特性及粒径等指标，探究该乳液的稳定性及作用机制。【结果】APPC具有独特的球-链结构且界面稳定性较强。以APPC制备的白藜芦醇Pickering乳液可形成稳定的蛋白质-多糖三维网络结构，因此该乳液具有界面膜增强和空间位阻协同稳定机制，且表现出良好的pH稳定性、热稳定性和贮存稳定性。【结论】APPC可通过界面膜增强和空间位阻效应抑制液滴聚集，提高Pickering乳液的稳定性。该研究可为天然多糖类Pickering乳化稳定剂的开发与应用提供参考。

Abstract:

[Objective] The present study prepared the Auricularia auricula protein-polysaccharide complex (APPC) with Auricularia auricula as the raw material and then prepared and characterized resveratrol-loaded Pickering emulsions based on APPC, aiming to investigate the stability and functioning mechanisms of resveratrol-loaded Pickering emulsions based on APPC. [Method] APPC was obtained via the water extraction and alcohol precipitation method, and its composition and structure were systematically analyzed. Furthermore, resveratrol-loaded Pickering emulsions were fabricated. The stability and functioning mechanisms of the emulsions were investigated based on the Zeta potential, three-phase contact angle, rheological properties, and particle size distribution. [Result] APPC possessed a unique sphere-chain structure with high interfacial stability. The resveratrol-loaded Pickering emulsions based on APPC exhibited a stable 3D network structure formed by proteins and polysaccharides. Therefore, the emulsions showed a synergistic stabilization mechanism of enhanced interfacial film and steric hindrance, with favorable pH stability, thermal stability, and storage stability. [Conclusion] APPC stabilizes Pickering emulsions through interfacial film reinforcement and steric hindrance effects. This study can provide a reference for the development and application of polysaccharide-based Pickering emulsions as stabilizers.