黑参皂苷超声波-酶法转化及免疫功效研究

Ultrasound-assisted Enzymatic Transformation and Immune Efficacy of Black Ginseng Saponins

摘要:

【目的】阐明黑参皂苷的转化工艺及其免疫调节机制。【方法】以黑参为原料，采用超声波-酶法对黑参皂苷组分进行转化，并测定黑参皂苷质量浓度；同时通过动物试验探究黑参皂苷的免疫调节功效。【结果】在酶解温度40 ℃、酶添加质量分数8%、酶解时间60 min、超声时间25 min的条件下，黑参皂苷质量浓度由1 615.40 μg/mL提高至3 351.38 μg/mL，增幅达107.47%。动物试验结果表明，黑参皂苷能够显著提高小鼠体质量、脾脏指数及胸腺指数，并显著提升血清中免疫球蛋白IgM、IgG和IgA的水平。Western blot分析进一步揭示，黑参皂苷可显著上调蛋白质NOD1、NOD2、RIP2、TAK1、IKKα/β及NF-κB的表达。【结论】该研究可为超声波-酶法转化黑参皂苷及其免疫功效研究提供理论参考。

Abstract:

[Objective]This study aims to elucidate the transformation process of black ginseng saponins and their immunomodulatory mechanisms.[Method]An ultrasound-assisted enzymatic method was employed for the transformation of black ginseng saponins, and the mass concentration of black ginseng saponins was determined. Furthermore, the immunomodulatory effects of black ginseng saponins were investigated through animal experiments.[Result]Under the conditions of an enzymatic hydrolysis temperature of 40 ℃, an enzyme mass fraction of 8%, enzymatic hydrolysis time of 60 min, and ultrasonic treatment time of 25 min, the mass concentration of black ginseng saponins increased from 1 615.40 μg/mL to 3 351.38 μg/mL, which represented an increase of 107.47%. The results of animal experiments showed that black ginseng saponins significantly increased the body mass, spleen index, and thymus index of mice, and significantly elevated the serum levels of immunoglobulins IgM, IgG, and IgA. Western blot analysis further revealed that black ginseng saponins significantly upregulated the expression of NOD1, NOD2, RIP2, TAK1, IKKα/β, and NF-κB.[Conclusion]This study provides a theoretical reference for the ultrasound-assisted enzymatic transformation of black ginseng saponins and the research on their immunomodulatory effects.