益生菌产品中菌群组成快速鉴别技术研究进展

Research Progress in Rapid Identification Technologies for Community Composition in Probiotic Products

摘要:

益生菌对人体健康具有多种促进作用，目前已广泛应用于食品、医药等领域。益生菌的功效高度依赖于产品中特定的微生物群落组成，因此，快速、准确的菌群组成鉴别技术对产品质量控制、功效评估与市场监督管理具有重要支撑作用。作者将现有鉴别方法划分为基于培养的方法、单细胞分析技术、分子生物学技术3类，综述了各类技术的原理、具体分析内容（如活菌计数、群落多样性解析），同时指出当前技术所面临的挑战，并对未来研究方向进行了展望，旨在为益生菌产品质量控制及相关领域的发展提供参考。

Abstract:

Probiotics exert multiple beneficial effects on human health and have been widely applied in fields such as food and medicine. Their efficacy is highly dependent on the specific microbial community composition in the products. Therefore, rapid and accurate identification technologies for community composition play a crucial supporting role in product quality assurance, efficacy control, and market supervision and management. According to technical principles, the author classifies existing identification methods into three categories: culture-based methods, single-cell analysis technologies, and molecular biology technologies. The author elaborates on the principles and specific analytical contents (such as viable count and community diversity analysis) of each category of technologies. Meanwhile, the author points out the challenges currently faced by these technologies and provides prospects for future research directions, aiming to offer a reference for the quality control of probiotic products and the development of related fields.