唾液联合乳杆菌CCFM1398及其后生元对大鼠正畸诱导性骨吸收的影响

Effects of Ligilactobacillus salivarius CCFM1398 and Its Postbiotics on Orthodontic-induced Bone Resorption in Rats

摘要:

【目的】评估唾液联合乳杆菌（Ligilactobacillus salivarius, L. salivarius）CCFM1398及其后生元对大鼠正畸诱导性骨吸收的缓解作用。【方法】采用MC3T3-E1成骨细胞模型和正畸诱导性骨吸收大鼠模型，综合评价唾液联合乳杆菌CCFM1398及其后生元调节牙槽骨骨吸收的生物活性。【结果】在MC3T3-E1细胞模型中，唾液联合乳杆菌CCFM1398能够显著上调骨保护素（osteoprotegerin，OPG）的表达。在大鼠模型中，该菌株或其后生元能够显著降低探诊深度和牙龈指数评分，并通过减少破骨细胞数量改善骨吸收，同时下调牙龈组织中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-6（interleukin-6，IL-6）和髓过氧化物酶（myeloperoxidase，MPO）的表达水平，有效缓解牙龈炎症。【结论】证实唾液联合乳杆菌CCFM1398及其后生元可有效改善大鼠正畸诱导性骨吸收，为开发具有骨吸收调节功能的益生菌/后生元制剂提供理论依据。

Abstract:

[Objective]This study aims to evaluate the alleviating effects of Ligilactobacillus salivarius (L. salivarius)CCFM1398 and its postbiotics on orthodontic-induced bone resorption in rats.[Method]This study used MC3T3-E1 osteoblast model and the rat model of orthodontic-induced bone resorption to comprehensively evaluate the biological activity of L. salivarius CCFM1398 and its postbiotics in modulating alveolar bone resorption.[Result]L. salivarius CCFM1398 significantly induced the expression of osteoprotegerin (OPG) in MC3T3-E1 cells. This strain or its postbiotics significantly reduced the probing depth and gingival index score of the rat model of orthodontic-induced bone resorption. They ameliorated bone resorption by reducing the number of osteoclasts and relieved gingival inflammation by down-regulating the expression levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and myeloperoxidase (MPO).[Conclusion]L. salivarius CCFM1398 and its postbiotics can ameliorate orthodontic-induced bone resorption in rats. The findings provide a theoretical basis for the development of probiotic/postbiotic agents with bone resorption-modulating functions.