山楂水提物延缓果蝇衰老行为的研究

Anti-aging Effect of Hawthorn Water Extract on Drosophila melanogaster

摘要:

【目的】以野生型黑腹果蝇为模式生物，探讨山楂水提物对果蝇衰老行为的改善作用。【方法】将果蝇分为对照组（基础培养基）和试验组（添加山楂水提物的培养基），通过测定果蝇寿命、垂直攀爬能力、繁殖能力、抗热应激能力及体内丙二醛（MDA）质量摩尔浓度，分析山楂水提物的抗衰老能力。【结果】与对照组相比，试验组雄性果蝇延寿率达25.06%，试验组果蝇的垂直攀爬能力与抗热应激能力均显著提高（P<0.05），MDA质量摩尔浓度显著降低（P<0.05）。此外，山楂水提物可有效延长果蝇寿命、改善机体功能，且对果蝇繁殖能力无显著影响。【结论】山楂水提物可有效延长果蝇寿命、延缓衰老行为，且无明显生殖毒性。该研究为探究其抗衰老作用机制奠定了基础，同时为山楂水提物在健康食品的开发应用提供了参考。

Abstract:

[Objective] This study aimed to explore the ameliorating effects of hawthorn water extract on aging-related behaviors in wild-type Drosophila melanogaster, the model organism. [Methods] Drosophila melanogaster individuals were allocated into two groups: a control group (basal medium) and an experimental group (basal medium + hawthorn water extract). The lifespan, vertical climbing ability, reproductive ability, heat stress resistance, and malondialdehyde (MDA) molality of Drosophila melanogaster were measured to analyze the anti-aging ability of hawthorn water extract. [Result] Compared with the control group, the experimental group showed a 25.06% increase in lifespan in males, enhanced vertical climbing ability and heat stress resistance in both genders (P<0.05), and a decrease in MDA molality (P<0.05). Moreover, the hawthorn water extract effectively prolonged the lifespan and delayed aging in Drosophila melanogaster without significantly compromising reproductive ability. [Conclusion] The hawthorn water extract can prolong the lifespan and delay the aging of Drosophila melanogaster, with low reproductive toxicity. This study lays a foundation for deciphering its anti-aging mechanism and provides a reference for the development and application of hawthorn water extract in health foods.