油橄榄果渣提取物对小鼠的抗疲劳作用研究

Anti-fatigue Effects of Olive Pomace Extracts in Mice

摘要:

【目的】评估油橄榄果渣（OP）提取物对小鼠的抗疲劳作用。【方法】对5个不同品种油橄榄的果渣进行酚类物质富集处理，采用高效液相色谱（HPLC）进行定量检测与分析。通过对小鼠力竭游泳时间、前肢握力和生化指标进行分析，系统评估OP提取物的抗疲劳效果。【结果】‘戈达尔’品种的OP提取物具有最高的总酚质量分数和抗氧化活性。动物试验结果显示，OP提取物可显著延长小鼠的力竭游泳时间、增强前肢握力，同时降低小鼠血清氨与血糖水平，上调血尿素氮（BUN）和血清肌酸激酶（CK）水平，表明该提取物可有效促进运动后肌肉恢复，缓解运动诱导的代谢应激。此外，组织病理学结果表明，补充OP提取物未对小鼠主要脏器造成损伤，具备良好的安全性。【结论】该研究为OP的高值化利用提供了科学依据，同时为研发天然安全的抗疲劳补充剂提供了技术参考。

Abstract:

[Objective] The study aimed to evaluate the anti-fatigue effects of olive pomace (OP) extracts in mice. [Method] Phenolic compounds were enriched from the pomace of five olive varieties and quantified and analyzed via high-performance liquid chromatography (HPLC). The anti-fatigue effects of OP extracts in mice were systematically assessed through exhaustive swimming, forelimb grip strength, and biochemical indicator. [Result] The OP extract of ‘Gordal’ had the highest total phenol mass fraction and antioxidant activity. The animal test results demonstrated that OP extracts significantly prolonged exhaustive swimming time, enhanced forelimb grip strength, and reduced serum ammonia and blood glucose levels while increasing blood urea nitrogen (BUN) and serum creatine kinase (CK) levels, suggesting its potential to improve muscle recovery after exercise and alleviate exercise-induced metabolic stress. Furthermore, histopathological examinations revealed no organ damage caused by OP extract supplementation. [Conclusion] This study not only provides scientific evidence for the high-value-added utilization of OP but also offers new technical insights into the development of natural safe supplements with anti-fatigue effects.