CRISPR/Cas系统在细菌性病原体检测中的研究进展

Research Progress of CRISPR/Cas Systems in Detection of Bacterial Pathogens

摘要:

细菌性传染病长期以来对全球公共卫生构成严重挑战。传统的病原体检测方法虽已在临床应用中取得了一定成果，但存在灵敏度低、检测周期长等缺陷。CRISPR/Cas系统作为一种新型的基因编辑工具，凭借其高效、精确的特性被广泛应用于病原体检测领域。作者概述了CRISPR/Cas系统的技术原理及其优缺点，并综述了近年来利用CRISPR/Cas技术检测不同细菌属的研究进展，以期为细菌性病原体的检测提供参考。

Abstract:

Bacterial infectious diseases have long posed a serious challenge to global public health. Although conventional pathogen detection methods have made some achievements in clinical application, they still face the limitations of low sensitivity, long time consumption and so on. As new gene editing tools, CRISPR/Cas systems are widely used in pathogen detection because of the high efficiency and accuracy. The author summarized the technical principle, advantages, and disadvantages of CRISPR/Cas systems, and briefly reviewed the research progress of using CRISPR/Cas technology to detect different bacterial genera in recent years, aiming to provide reference for the detection of bacterial pathogens in the future.