快速测试片法在冷鲜肉微生物定量检测中的应用

Application of a Rapid Test Strip Method in Quantitative Detection of Microorganisms in Chilled Meat

摘要:

【目的】旨在评估快速测试片法用于冷鲜肉中微生物质量控制中的关键检测指标菌落总数、大肠杆菌/大肠菌群检测的可行性。【方法】采用重复性试验验证快速测试片的性能，并以国家标准中平板计数法为对照进行方法比对。采用t检验评估2种方法检测结果的显著性，并通过线性回归分析其相关性。【结果】快速测试片法的重复性偏差r≤0.25，符合ISO 4833—1：2013的要求，与平板计数法的检测结果无显著性差异，两者呈高度正相关。【结论】快速测试片法具备良好的重复性与准确性，可用于冷鲜肉中菌落总数、大肠杆菌/大肠菌群的快速检测，为冷鲜肉的微生物安全监测提供技术支持。

Abstract:

[Objective] This study aims to evaluate the feasibility of using the rapid test strip method for detecting key microbial quality control indicators in chilled meat, specifically the total plate count and Escherichia coli/coliforms.[Method] The performance of the rapid test strip method was assessed through repeatability tests, and the national standard plate count method was used as the reference for method comparison. Statistical significance of the quantification results was analyzed by a t-test, and linear regression was adopted to analyze the correlation.[Result] The repeatability deviation of the rapid test strip method (r≤0.25) met the requirements of ISO 4833—1:2013. No significant difference was observed between the results of the rapid test strip method and the plate count method, and the results of the two methods demonstrated a positive correlation.[Conclusion] The rapid test strip method demonstrates good repeatability and accuracy, and thus it can be used for the rapid quantification of the total plate count and Escherichia coli/coliforms in chilled meat, providing technical support for microbial safety monitoring in chilled meat.