银杏叶提取物和水溶性番茄浓缩物复合剂的降血脂功效研究

Lipid-lowering Effect of Ginkgo biloba Leaf Extract and Water-soluble Tomato Concentrate Complex

摘要:

目的 探究银杏叶提取物（GBE）和水溶性番茄浓缩物（WSTC）复合剂对高脂血症（HLP）大鼠的影响。方法 选取40只SD大鼠，10只作为空白对照（CON）组，其余30只通过建立高脂模型后随机分为模型对照（MOD）组、阳性对照（PC）组和GBE+WSTC复合剂组。给药45 d后，测定血清中血脂指标：总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）的浓度；机理指标：低密度脂蛋白受体（LDLR）、羟甲基戊二酸单酰辅酶A还原酶（HMG-CoAR）和胆固醇7-羟化酶（CYP7A1）的质量浓度；抗氧化指标：丙二醛（MDA）、总超氧化物歧化酶（T-SOD）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）水平。结果 与MOD组相比，在血脂指标中，GBE+WSTC复合剂组血清中TC和LDL-C的浓度极显著降低（P<0.01），TG浓度极其显著降低（P<0.001），而HDL-C浓度无显著差异（P>0.05）；在机理指标中，LDLR质量浓度显著升高（P<0.05），HMG-CoAR质量浓度显著降低（P<0.05），CYP7A1质量浓度极其显著升高（P<0.001）；在抗氧化指标中，MDA浓度极其显著降低（P<0.001），SOD酶活力极其显著升高（P<0.001），而GSH质量浓度和GSH-Px酶活力无显著差异（P>0.05）。结论 GBE+WSTC复合剂组可有效改善HLP大鼠的症状，该研究为降血脂功能研究提供了理论依据。

Abstract:

Objective This study aims to investigate the effect of the Ginkgo biloba leaf extract (GBE) and water-soluble tomato concentrate (WSTC) complex on hyperlipidemia (HLP) rats.Method From 40 SD rats, 10 rats were selected as blank control (CON) group, and the remaining 30 rats were modeled for HLP and then randomly allocated into model control (MOD), positive control (PC), and GBE+WSTC complex groups. After 45 d of administration, serum samples were collected for the measurement of the levels of lipid indicators: concentrations of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C); mechanism indicators were determined: mass concentrations of low-density lipoprotein receptor (LDLR), hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoAR), and cholesterol 7-hydroxylase (CYP7A1); antioxidant indicators were assayed: levels of malondialdehyde (MDA), total superoxide dismutase (T-SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px).Result Compared with the MOD group, the GBE+WSTC complex group showed highly significant reducations in the concentrations of TC and LDL-C in the serum (P<0.01), and an extremely significant decrease in the concentration of TG (P<0.001), while there was no significant difference in the concentration of HDL-C (P>0.05). Among the mechanism indicators, the mass concentration of LDLR was significantly increased (P<0.05), while the mass concentration of HMG-CoA was significantly decreased (P<0.05) in the GBE+WSTC complex group in comparison with the MOD group. Particularly, the mass concentration of CYP7A1 showed an extremely significant increase (P<0.001). In terms of antioxidant indicators, the GBE+WSTC complex group showed an extremely significant declined concentration of MDA (P<0.001), an extremely significant enhanced activity of SOD (P<0.001), and no significant difference in GSH and GSH-Px (P>0.05).Conclusion The GBE+WSTC complex can effectively alleviate the symptoms of HLP in rats. The findings provide a theoretical basis for the study of the blood lipid-lowering function.