基于CRISPR/Cas系统的生物传感器在食源性致病菌检测中的应用

Application of CRISPR/Cas System-based Biosensors in Detection of Foodborne Pathogens

摘要:

CRISPR/Cas系统可识别外源DNA，切割靶标并阻止沉默外源基因的表达。由于精确的靶向功能，CRISPR/Cas系统被开发成1种高效的基因编辑工具。基于其高度的特异性及可操作性，将其与生物传感器联用，提高了传感器的检测特异性及效率。作者介绍了CRISPR/Cas系统的特点和应用，分析了基于CRISPR/Cas系统的生物传感器在食源性致病菌检测方面的最新进展并对其应用进行了总结和展望。

Abstract:

The CRISPR/Cas system can recognize foreign DNA, cut the target, and block or silence the expression of the foreign gene. Due to its precise targeting function, the CRISPR/Cas system has been developed as a highly efficient gene editing tool. With high specificity and operability, the CRISPR/Cas system has been combined with biosensors to improve the detection specificity and efficiency of the sensors. This review introduces the characteristics and applications of CRISPR/Cas system, analyzes the latest progress in the application of CRISPR/Cas system-based biosensors in the detection of foodborne pathogens, and summarizes and prospects the application of such biosensors.