脂肪酶酶活力检测方法研究进展

Research Progress of Lipase Enzyme Activity Detection Methods

摘要:

脂肪酶是一种关键的生物催化剂，在催化甘油三酯水解和脂肪酸酯化过程中发挥着重要作用，因此准确测定脂肪酶酶活力至关重要。传统方法中的平板检测法和滴定法是通过测定脂肪酶催化底物产生的脂肪酸来间接评估脂肪酶酶活力，而铜皂比色法、对硝基苯酚法和探针比色法则依据产物颜色的变化来测定脂肪酶酶活力。近年来出现的新兴方法中的液体泡沫检测法只能用于定性分析；相比之下，荧光探针法和基于生物传感器的检测方法灵敏度高，更适于复杂介质中脂肪酶酶活力的检测。作者简述了脂肪酶酶活力检测方法的原理、存在问题、适用条件和发展现状，并对脂肪酶酶活力检测方法进行了总结和对比，旨在为脂肪酶酶活力的检测研究提供参考。

Abstract:

As key biocatalysts, lipases play essential roles in catalyzing the hydrolysis of triglycerides and the esterification of fatty acids. Therefore, accurate measurement of lipases enzyme activity is crucial. Traditional methods, such as plate assay and titration, indirectly assess lipase enzyme activity by measuring the fatty acids produced from the substrate catalyzed by lipases. Methods like the copper-soap colorimetric assay, p-nitrophenol assay, and probe colorimetric assay determine lipase enzyme activity based on changes in the color of the reaction products. Recently developed methods, such as liquid foam detection, are limited to qualitative analysis, whereas the fluorescence probe and biosensor-based detection methods offer higher sensitivity, making them more suitable for measuring lipase enzyme activity in complex media. The author provides a brief overview of the principles, limitations applicable conditions, and current developments of various lipase enzyme activity detection methods, along with a comprehensive comparison and summary, aiming to offer a reference for future research on lipase enzyme activity detection.