分子印迹电化学传感器NH2-MWCNTs@4-FPBA检测乳品中表皮生长因子的方法建立

Establishment of a Molecularly Imprinted Electrochemical Sensor NH2-MWCNTs@4-FPBA for the Detection of Epidermal Growth Factor in Dairy Products

摘要:

【目的】构建一种快速、准确的表皮生长因子（EGF）检测方法。【方法】将4-甲酰基苯硼酸（4-FPBA）共价锚定到氨基碳纳米管（NH₂-MWCNTs）表面，合成纳米复合材料（NH₂- MWCNTs@4-FPBA），将NH₂-MWCNTs@4-FPBA修饰于玻碳电极上，当存在EGF时，4-FPBA通过苯甲醛基捕获EGF模板，再将电极浸入吡咯溶液中，电聚合形成吡咯分子印迹层，最后在酸性溶液中解离去除EGF模板后，即可形成与EGF模板互补的三维空腔。【结果】该传感器具有0.45~500.00 pg/mL的宽线性范围和0.45 pg/mL的低检测限，并具有良好的选择性、重复性和稳定性。【结论】利用该分子印迹电化学传感器检测出酸奶中EGF质量浓度为3.67 pg/mL，超高温瞬时灭菌牛乳中EGF质量浓度为29.03 pg/mL。

Abstract:

[Objective]This study aims to develop a rapid and accurate method for detecting epidermal growth factor (EGF).[Method]4-Formylphenylboronic acid (4-FPBA) was covalently anchored to the surface of aminocarbon nanotubes (NH₂-MWCNTs) to synthesize nanocomposites (NH₂-MWCNTs@4-FPBA), and NH₂-MWCNTs@4-FPBA was modified on a glassy carbon electrode. When EGF was present, 4-FPBA captured the EGF template through benzaldehyde, and then the electrode was immersed in pyrrole solution to electropolymerize to form a pyrrole molecularly imprinted layer. Finally, after dissociation and removal of the EGF template in an acidic solution, a three-dimensional cavity complementary to the EGF template was formed.[Result]The sensor had a wide linear range of 0.45~500.00 pg/mL and a low detection limit of 0.45 pg/mL, with excellent selectivity, reproducibility, and stability.[Conclusion]The measurement using this molecularly imprinted electrochemical sensor showed that the mass concentration of EGF was 3.67 pg/mL in yoghurt and 29.03 pg/mL in ultra-high temperature transiently sterilized cow milk.