ZIF-8固定化酶电极对食品中葡萄糖溶液的检测

A ZIF-8 Electrode Immobilizing Enzyme for Detection of Glucose Solution in Food

摘要:

【目的】构建基于金属有机骨架修饰电极的电化学传感器，实现对饮料中葡萄糖溶液的高灵敏检测。【方法】通过原位合成法和固定化酶技术制备一种基于ZIF-8复合碳纳米管材料并固载葡萄糖氧化酶（GOx）的电化学传感器，使用X射线衍射仪（XRD）、扫描电镜（SEM）、光谱学仪器紫外（UV-Vis）和红外（IR）表征材料的微观结构和形貌，并结合电化学工作站对固定化酶电极进行电化学性能测试。【结果】制备的固定化酶电极对葡萄糖溶液展现出优异的检测性能，检出限为1.395 μmol/L。【结论】该传感器在食品检测领域具有良好的应用前景。

Abstract:

[Objective]The aim was to construct an electrochemical sensor based on a metal-organic framework-modified electrode for the highly sensitive detection of glucose solution in beverages.[Method]An electrochemical sensor based on ZIF-8 composite carbon nanotubes and immobilizing glucose oxidase (GOx) was prepared by in-situ synthesis and immobilized enzyme technology. The microstructure and morphology of the material were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-Vis spectroscopy, and infrared spectrometry (IR). The electrochemical performance of the electrode immobilizing enzyme was tested by electrochemical workstation.[Result]The prepared electrode immobilizing enzyme showed excellent detection performance for glucose solution, with a limit of detection being 1.395 μmol/L.[Conclusion]The sensor has a good application prospect in the field of food detection.