植物甾醇油脂体乳液对秀丽隐杆线虫脂代谢的影响及机制研究

Effects and Mechanisms of Phytosterol-oil Bodies Emulsion on Lipid Metabolism of Caenorhabditis elegans

摘要:

【目的】研究植物甾醇油脂体乳液（phytosterol-oil bodies emulsion，POE）的降脂作用。【方法】以秀丽隐杆线虫为模型，测定POE处理后线虫的脂代谢和生理相关指标，并分析线虫氧化应激和脂肪代谢相关基因水平变化。【结果】与对照组相比，不同质量浓度POE给药组可显著降低线虫肠道脂肪质量分数（P<0.01），显著降低甘油三酯的相对含量（P<0.05），对线虫生长发育无毒害作用；同时，还能显著降低活性氧水平，提高抗氧化酶水平；影响与脂肪代谢相关基因表达水平，qPCR检测结果显示，sbp-1基因mRNA水平上调，fat-2、nhr-80、fat-5 mRNA水平下调（P<0.05）。【结论】POE可能通过减缓脂肪沉积、调节脂肪过氧化途径和减轻线虫体内氧化应激水平来改善脂质代谢。

Abstract:

[Objective]This study aims to explore the lipid-lowering effects of the phytosterol-oil bodies emulsion (POE).[Method]Caenorhabditis elegans was taken as a model to evaluate the effects of POE on lipid metabolism, physiological indexes, and expression of genes associated with oxidative stress and fat metabolism.[Result]Compared with the control group, different mass concentrations of POE reduced the mass fraction of fat (P<0.01) and the relative content of triglycerides (P<0.05), without toxicity to the growth and development of C. elegans (P<0.01). At the same time, it significantly reduced the level of reactive oxygen species and increased the levels of antioxidant enzymes. In addition, POE affected the expression levels of genes related to fat metabolism. qPCR results showed that POE up-regulated the mRNA level of sbp-1, while down-regulating the mRNA levels of fat-2, nhr-80, and fat-5 (P<0.05).[Conclusion]POE may improve lipid metabolism by slowing fat deposition, regulating the fat peroxidation pathway, and alleviating oxidative stress in C. elegans.