荧光微球免疫分析法快速测定婴幼儿配方乳粉中的维生素B1

Rapid Determination of Vitamin B1 in Infant Formula Milk Powder by Fluorescent Microsphere Immunochromatographic Assay

摘要:

【目的】基于维生素B₁单克隆抗体建立荧光免疫层析法，以检测婴幼儿配方乳粉中的维生素B₁。【方法】对维生素B₁单克隆抗体的性能进行评估，在此基础上，开发具有高灵敏度的荧光免疫层析试纸条。【结果】维生素B₁单克隆抗体具有高灵敏度、高亲和力和高特异性，荧光免疫层析试纸条的视觉检测限（vLOD）为10 μg/kg，消线值为200 μg/kg，计算检测限（cLOD）为2.056 μg/kg。此外，该方法的回收率为97.3%~115.4%，与液相色谱-串联质谱法（LC-MS/MS）检测结果一致，且相对标准偏差（RSD）小于10%，具有较好的准确度和稳定性。【结论】该荧光微球免疫分析法可用于实际婴幼儿配方乳粉样本的检测，并且检测速度快，不需要大型仪器设备，便于携带，对于大量样本的快速筛检具有重要意义。

Abstract:

[Objective] A fluorescent immunochromatographic assay was established based on vitamin B₁ monoclonal antibody to measure the vitamin B₁ in infant formula milk powder.[Method] The performance of vitamin B₁ monoclonal antibody was evaluated. On this basis, a highly sensitive fluorescent immunochromatographic test strip was developed.[Result] The vitamin B₁ monoclonal antibody showed high sensitivity, affinity, and specificity. The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD) of 10 μg/kg, a cut-off value of 200 μg/kg, and a calculated limit of detection (cLOD) of 2.056 μg/kg. In addition, the recovery rate of this method was 97.3%~115.4%. The test results of the established method were consistent with the liquid chromatography-tandem mass spectrometry (LC-MS/MS) results, with the relative standard deviation (RSD) less than 10%, which indicated good accuracy and stability.[Conclusion] The fluorescent immunochromatographic assay can be used for the rapid detection of actual infant formula milk powder samples. It does not require large-scale instruments or equipment and is portable, thus being of great significance for the rapid screening of a large number of samples.