用于卡那霉素检测的竞争性比色传感策略研究

Research on Competitive Colorimetric Sensing Strategy for Kanamycin Detection

摘要:

由于畜牧业和渔业等食品相关领域抗生素的使用不当对环境和人体造成的严重影响已经引起了众多研究者的广泛关注。作者制备了磁珠-卡那霉素这一磁性复合物，通过磁珠表面的卡那霉素与待测样中的卡那霉素共同竞争识别体系中的适配体，开发了一种简单、快速、灵敏的竞争性比色适配体传感器。通过扫描电子显微镜和紫外-可见分光光度计对磁珠-卡那霉素的合成及适配体与靶标之间的生物识别进行表征。这两者的成功为该传感策略的实施提供了有力保障。该传感器在卡那霉素浓度为0.05~500 nmol/L的检测范围内有良好的比色效能，检测限为0.21 nmol/L。另外，通过与其他氨基糖苷类抗生素的检测实验对比，可以得出该传感器对卡那霉素的选择性良好。总之，该比色适配体传感器操作简单，无需大型仪器，为其他抗生素以及影响食品安全的其他化学污染物的快速检测提供了良好思路。

Abstract:

The serious impact of the improper use of antibiotics in food-related fields such as animal husbandry and fishery on the environment and human body has attracted widespread attention of many researchers. In this study, a magnetic beads-kanamycin complex was prepared, and a simple, rapid and sensitive competitive colorimetric aptamer sensing strategy was developed by competing for the aptamer in the system between kanamycin on the surface of magnetic beads and kanamycin in the sample. The synthesis of magnetic beads-kanamycin and the biological recognition between the aptamer and the target were characterized by scanning electron microscope and ultraviolet-visible spectrophotometer. The successful synthesis and recognition provide a strong guarantee for the sensing strategy construction. The sensor has good colorimetric performance in the detection range of kanamycin molar concentration of 0.05~500 nmol/L, and the limit of detection is 0.21 nmol/L. In addition, by comparing with other aminoglycoside antibiotics detection experiment, it could be concluded that the sensor has a good selectivity to kanamycin. In short, the colorimetric aptasensor is easy to operate without large-scale instruments, providing a good idea for the rapid detection of other antibiotics and other chemical contaminants affecting food safety.