蛋清酶水解工艺优化及水解物对小鼠生理指标的影响

Study on the Optimal Hydrolysis Condition of Egg White and Effect of Protein Hydrolysate on Physiological Indexes of Mice

摘要:

通过正交试验得出Neutrase 蛋白酶水解蛋清的最佳条件，然后选取2月龄雄性小鼠40只，随机分成4组，每组10只，连续灌喂蛋清水解物4周，检测小鼠的血清生化指标和肝脏抗氧化指标。结果表明，酶水解的优化条件为：温度55 ℃、pH 6.0、酶量为5 g/dL、蛋清体积比为1∶5。在此条件下，水解物的蛋白质残留率为24.28%，水解度达3.71%。水解物的相对分子质量大部分集中在1 000以下，其中180~300、300~500、500~1 000所占的比率分别为28.03%、41.84%、23.69%。N?鄄6h组小鼠血清中的谷丙转氨酶、葡萄糖和总胆固醇含量显著低于对照组（P<0.05）。N-3h组小鼠肝脏的T-SOD和GSH-PX活力显著高于对照组（P<0.05）；N?鄄6h组小鼠肝脏的MDA含量显著低于对照组（P<0.05）。综上，蛋清水解物能够改善小鼠生理功能，其中酶解3 h时的产物效果较好。

Abstract:

To obtain the optimal hydrolysis condition of egg white by Neutrase enzyme，forty 2-month male mice were divided into 4 groups，control group，egg white protein group，N-6h group and N-3h group，respectively. The mice were injected with 0.3mL egg white peptide by gavage. The serum biochemical indexes and liver anti-oxidase vitality were measured after four weeks. The results showed that the optimal hydrolysis technology of were as follows，temperature at 55 ℃，pH 6.0，5 % of enzyme dosage and concentration of egg white for 1∶5. Under this condition，the residual rate and hydrolyzing degree of protein were 24.28 %and 3.71%，respectively. The molecular weight of hydrolysate were mainly less than 1 000，the percentage of 180~300，300~500 and 500~1000 were 28.03%，41.84% and 23.69%，respectively. The serum glutamic-pyruvic transaminase，glucose ，total cholesterol content of mice and the liver MDA content in N-6h group were lower and the liver T-SOD and GSH-PX vitality of mice in N-3h group were higher as compared to the control group（P<0.05）.In conclusion，the hydrolysate of egg white could improve physiological function of mice，and the effect of 3h enzymolysis product was better.