硝氮质量浓度和温度在不同培养模式下对雨生红球藻生长的影响

Research on the Effects of Nitric Nitrogen and Temperature on Heamatococcus lacustris Under Different Cultural Modes

摘要:

探究了自养和异养条件下，硝氮质量浓度和生长温度对雨生红球藻生长的影响，以及自养胁迫阶段不同胁迫温度（30，35，38 ℃）对虾青素积累的影响。研究结果表明：雨生红球藻在异养条件下的最高生物量（3.319 g/L）远高于自养条件下最高生物量（0.687 g/L），生物量提高了84%。自养条件下虾青素最高质量分数为2.264%，异养条件下虾青素最高质量分数为3.199%，比自养条件下虾青素最高质量分数提高41.29%。异养生长具有明显的生长优势，此条件下最适碳源为乙酸钠质量浓度1.5 g/L，最适硝氮质量浓度浓度为0.5 g/L，最适生长温度为22 ℃，最佳胁迫温度为30 ℃。

Abstract:

Autotrophic and heterotrophic growth and the effect of nitric nitrogen，temperature on Heamatococcus lacustris were investigated. The microalgae grew better in heterotrophic mode. In autotrophic and heterotrophic the biomass were 0.687 g/L and 3.319 g/L respectively ，the astaxathin content were 2.264% and 3.199%，respectively. In heterotrophic，the optimum growth concentration of sodium acetate was 1.5 g/L，nitric nitrogen was 0.5 g/L，growth temperature was 22 ℃，the suitable stress temperature was 30 ℃.