利用枯草芽孢杆菌发酵生产环磷腺苷的工业化试验

Process of Adenosine-3',5'-Cyclic Monophosphate Production by Fermentation with Bacillus. subtilis

摘要:

为了实现环磷腺苷的发酵生产, 对一株枯草芽孢杆菌 Bacillus. subtilis jsim-1277在摇瓶、自控罐、20 M3发酵罐上的发酵条件进行了试验。结果表明, 最适宜发酵条件是pH 6.7, 且添加K2HPO4 1.0 g/dL, NaF 0.05 g/dL; 在摇瓶、自控罐、大型发酵罐产苷分别为7.17、6.01、7.10 g/L。

Abstract:

In order to prepare the adenosine-3', 5'-cyclic monophosphate by fenmetation, the ferment conditions of Bacillus. subtilis jsim-1277 were studied in shake flask, auto fermentor, and 20 M3 fermentor. The results showed that the optimal conditions for the fermentation were identified as follow：pH at 6.7, the optimal concentration of K2HPO4 was 1.0 g/dL, the optimal concentration of NaF was 0.05 g/dL. Under the optimized fermentation conditions, the productivity in shake flask, auto fermentor, or 20 M3 fermentor was 7.17, 6.01, 7.10 g/L, respectively.