高效液相色谱测定不同采收期罗布麻中黄酮含量

Simultaneous Determination of Four Flavonoids in Apocynum venetum Gathered in Different Seasons by High Performance Liquid Chromatography

摘要:

采用高效液相色谱法同时测定罗布麻叶中芦丁、异槲皮苷、金丝桃苷和槲皮素4种黄酮的含量,从而确定最佳的罗布麻采收期。样品采用超声波法提取。色谱条件为:色谱柱:Zorbax Eclipse C18(150 mm 4.6 mm,5μm);流动相:甲醇-0.4%磷酸水溶液(50∶50);流量1.0 mL/min;紫外检测波长360 nm;柱温:50℃。该方法对4种黄酮能够很好分离,线性关系、精密度、稳定性和加标回收率良好。按照这种方法检测出不同采收季节的罗布麻叶中4种黄酮含量,其中8月份采收的罗布麻叶中黄酮含量最高。高效液相色谱检测法操作简单快速、定量准确、灵敏度高、成本低,能很好地测定出罗布麻中黄酮的含量。

Abstract:

To develop a sensitive and specific high performance liquid chromatography(HPLC)method to determine four flavonoids in Apocynum venetum gathered in different seasons,so as to determine the optimal gathering season. The sample was extracted with ultrasonic method and then separated on Zorbax Eclipse C18(150 mm ×4.6 mm,5 μm) with a mixture of methanol-0.4%H3PO4(volume ratio 50∶50) as mobile phase at flow rate of 1 mL/min. The UV detective wavelength was 360 nm,and the column temperature was set at 50 ℃. With this method,four flavonoids were separated efficiently. The liner relation,stability and the recovery rate were all good. The four flavonoids were determined with this method. The flavonoids content of Apocynum venetum leaves gathered in August was the highest. The optimal gathering season of Apocynum venetum was August. The result indicated that the proposed method was simple,rapid,accurate,sensitive,and low cost,thus it provides an effective scientific means for assaying flavonoids in Apocynum venetum.