原生质体融合技术选育高效菌株降解聚乙烯醇

The Breeding of High Efficient Strain with the Technique of Protoplast Fusion to Degrade Polyvinyl-Alcohol

摘要:

从自然界中分离聚乙烯醇(PVA)的降解细菌，经紫外线诱变，得到两株具有单重抗药性的突变菌株S7(Str^s，Kan^r)和K15(Str^r，Kan^s)，两者对PVA的去除率分别达到52％和58％．将S7和K15作为亲本菌株进行原生质体融合，并对融合条件进行优化，融合子F4菌株对PVA去除率达到79．9％，将其培养成活性污泥后，PVA去除率可达87％，是普通活性污泥的三四倍．

Abstract:

Bacteria with the ability to degrade Polyvinyl-Alcohol(PVA), were isolated from natural specimens. Two single resistant mutants, S7(Str~(s)Kan~(r)) and K15(Str~(r)Kan~(s)) were obtained by UV-irradiation. The S7 strain and the K15 strain could remove 52%