微生物发酵合成15N-L-脯氨酸

Biosynthesis of 15N-L-Proline by Fermentation

摘要:

采用含有稳定同位素15N 氯化铵为主要氮源的专用发酵培养基配方和相应的提取精制条件,采用微生物直接发酵方法研制15N L 脯氨酸高丰度产品.结果显示,每克15N 氯化铵实际可得0.376g15N L 脯氨酸,提取精制得率最高达65.76%,产品15N丰度达98.28%,比原料下降1.23%.

Abstract:

()~( 15)N-L-Proline was prepared by fermentation in a specific nutrient medium containing (()~(15)N-ammonium) chloride and growth stimulants, and with subsequent isolation and purification of ()~(15)N-L-Proline from fermentation liquid. 0.376 gram ()~(