抗分解代谢阻遏纤维素酶变异株的选育

Screening of Cellulase Mutant Resistant to Catabolite Repression

摘要:

利用紫外线、亚硝基胍等对里氏木霉进行诱变处理 ,采用低剂量、多次复合诱变处理方法 ,以 2 脱氧葡萄糖作为降解产物阻遏物进行高效筛选 ,选育得到一株抗分解代谢阻遏的突变株 ,使纤维素酶活力提高了 3倍 .

Abstract:

In screening high production mutant of cellulase, Trichoderma reesei DWC was treated repeatedly in lower dosage with UV, NTG, and so on. After screened by selection for resistance to 2 deoxyglucose, the mutant T. Reesei GB6 4 was obtained. The cellulas