核黄素产生菌的补料发酵

Batch-fed Fermentation of Riboflavin Producing Strain E.ashbyii

摘要:

通过分析核黄素产生菌E .ashbyii在发酵过程中菌体量、糖质量浓度、pH值、核黄素质量浓度和粘度的变化及菌体形态与核黄素生物合成之间的联系 ,发现 pH值对菌体生长有显著的影响 ,菌体浓度过高会导致发酵液粘度过大 ,通风供氧困难 ,菌体活力下降 .采用先低浓度培养菌体 ,然后根据发酵液 pH值的变化进行多次适量补料 ,不仅可以控制菌体的过度增殖 ,缓解通风供氧不足造成的困难 ,而且还可以保持菌体活力 ,增加核黄素的产量 .

Abstract:

By analyzing biomass, saccharide, pH, riboflavin and viscosity of the broth during riboflavin producing strain E.ashbyii fermentation process and the relationship between the strain profile and riboflavin biosynthesis, it was found that pH has great effec